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cell proliferation dye efluor 450  (Thermo Fisher)


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    Thermo Fisher cell proliferation dye efluor 450
    Cell Proliferation Dye Efluor 450, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%E2%84%A2+cell+proliferation+dye+efluor%E2%84%A2+450/cell+proliferation+dye+efluor+670/pm40605638-371-5-10
    Average 90 stars, based on 1 article reviews
    cell proliferation dye efluor 450 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Behavioral-transcriptomic landscape of engineered T cells targeting human cancer organoids
    Article Snippet: Prior to co-culturing, TEGs were incubated with eBioscienceTM Cell Proliferation Dye eFluorTM 450 (referred to as eFluor-450; 1:4000; Thermo Fisher) in PBS for 10 min at 37 °C to fluorescently label all TEGs.

    Article Title: Partner, Neighbor, Housekeeper and Dimension: 3D versus 2D Glomerular Co-Cultures Reveal Drawbacks of Currently Used Cell Culture Models.
    Article Snippet: Confocal microscopy of 3D glomerular co-culture displays green fluorescent podocyte- derived VEGFA, mesangial cells labeled in blue with eBioscience™ Cell Proliferation Dye eFluor™ 450 and red fluorescent tdTomato-Farnesyl glomerular endothelial cell reporter cells.

    Article Title: Partner, Neighbor, Housekeeper and Dimension: 3D versus 2D Glomerular Co-Cultures Reveal Drawbacks of Currently Used Cell Culture Models.
    Article Snippet: In detail, glomerular endothelial cells stably expressing a tdTomato-Farnesyl reporter were used to label glomerular endothelial cells in red, mesangial cells labeled in green with the eBioscienceTM CFSE (ThermoFisher Scientific) and podocytes in blue with the eBioscienceTM Cell Proliferation Dye eFluorTM 450.

    Article Title: Partner, Neighbor, Housekeeper and Dimension: 3D versus 2D Glomerular Co-Cultures Reveal Drawbacks of Currently Used Cell Culture Models
    Article Snippet: In detail, glomerular endothelial cells stably expressing a tdTomato-Farnesyl reporter were used to label glomerular endothelial cells in red, mesangial cells labeled in green with the eBioscienceTM CFSE (ThermoFisher Scientific) and podocytes in blue with the eBioscienceTM Cell Proliferation Dye eFluorTM 450.

    Article Title: Partner, Neighbor, Housekeeper and Dimension: 3D versus 2D Glomerular Co-Cultures Reveal Drawbacks of Currently Used Cell Culture Models
    Article Snippet: Cell suspension of conditionally immortalized human podocytes was stained with 2 μM of the eBioscienceTM Cell Proliferation Dye eFluorTM 450 (Invitrogen, ThermoFisher Scientific, Waltham, MA, USA), and mesangial cells were labeled with 1 μM of the eBioscienceTM CFSE (Invitrogen, ThermoFisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.

    Article Title: Deconvolution of cancer methylation patterns determines that altered methylation in cancer is dominated by a non-disease associated proliferation signal
    Article Snippet: Staining with eBioscienceTM Cell Proliferation Dye eFluorTM 450 (Invitrogen) was used to asses cell proliferation in transduced leukaemia cell lines.

    Labeling:

    Article Title: The Class IIA Histone Deacetylase (HDAC) Inhibitor TMP269 Downregulates Ribosomal Proteins and Has Anti-Proliferative and Pro-Apoptotic Effects on AML Cells.
    Article Snippet: .. Cells were labeled with a cell proliferation dye (eBioscience™ Cell Proliferation Dye eFluor™ 450, Invitrogen™, Waltham, MA, USA) diluted 1:5000 in PBS (final concentration: 2 µM). .. Then, 1× 105 cells were seeded in 500 μL medium in a 48-well plate and treated with the desired concentrations of the appropriate HDAC inhibitors for 48 h. After harvesting, cells were manually counted using a Neubauer chamber and proliferation was assayed by flow cytometry on a BD FACS Canto II (BD Biosciences, San Jose, CA, USA).

    Concentration Assay:

    Article Title: The Class IIA Histone Deacetylase (HDAC) Inhibitor TMP269 Downregulates Ribosomal Proteins and Has Anti-Proliferative and Pro-Apoptotic Effects on AML Cells.
    Article Snippet: .. Cells were labeled with a cell proliferation dye (eBioscience™ Cell Proliferation Dye eFluor™ 450, Invitrogen™, Waltham, MA, USA) diluted 1:5000 in PBS (final concentration: 2 µM). .. Then, 1× 105 cells were seeded in 500 μL medium in a 48-well plate and treated with the desired concentrations of the appropriate HDAC inhibitors for 48 h. After harvesting, cells were manually counted using a Neubauer chamber and proliferation was assayed by flow cytometry on a BD FACS Canto II (BD Biosciences, San Jose, CA, USA).



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